Finding the Right Primers: Using NCBI for RT- PCR Primer Design. Specializing in the design and troubleshooting of q. PCR, mutagenesis, and cloning experiments at IDT, Scientific Applications Specialist, Adam Clore Ph. D, speaks about the intelligent selection of PCR primers. Topics include identifying transcript variants for your target of interest and selecting primers that amplify only specific transcripts. Adam also discusses the growing SNP database and the potential impact of SNPs on q.
PCR data, how to locate any SNPs that fall in your target amplicon, and the use of free NCBI and IDT tools to help you avoid them. About the Speaker: Adam Clore. Adam Clore is a Scientific Applications Specialist at Integrated DNA Technologies. He received his BS in Microbiology and Chemistry from the University of Northern Iowa and his Ph. D in Biology from Portland State University where he studied the molecular genetics of Hyperthermophilic Archaea and their viruses. His postdoctoral research involved studying DNA damage from environmental toxins and repair pathways. His roles at IDT include assay design and troubleshooting, new product development, research, and scientific writing.
- These software applications determine the properties of any oligo sequence entered, as well as facilitate the intelligent design of assay conditions, all at the click of a button.
- Primer design can sometimes feel like more of an art than a science, and designing the best primer can significantly affect the success or failure of your experiments. Here are a few tips on optimizing primer design for.
- Specializing in the design and troubleshooting of qPCR, mutagenesis, and cloning experiments at IDT, Scientific Applications Specialist, Adam Clore PhD, speaks about the intelligent selection of PCR primers.
- BACKGROUND INFORMATION: For sites describing PCR theory, as well as companies marketing PCR products you might want to begin by visiting Highveld. For PCR techniques see PCRlink.com.
- DNA Software is the preferred partner to solve your diagnostic design and analysis problems. We build customized software platforms and provide diagnostic solutions to our partners.
Free q. PCR Software, free qpcr design software, Beacon Designer Free Edition for free SYBR Green and Taq. Man Analysis. Beacon Designer. In addition to these two chemistries design support is available for Methy. Light, LNA, Molecular beacons, NASBA, FRET and Scorpion assays. Beacon Designer. Sequences are BLAST searched and folded, the results are automatically interpreted and used for designing primers.
This step ensures that the primers are highly specific and efficient. Beacon Designer. All sets are analyzed for cross compatibility and cross homology to other targets to ensure specific and efficient amplification. Please visit the Beacon Designer.
Our handy Oligo Analysis Tool calculates molecular weight (MW), extinction coefficient (E260), pmoles/. Also displayed for your convenience are the % GC Content, melting temp (TM), and. Learn to design, optimize, and validate real-time PCR assays. Primer and probe design, target selection, gradient, melt curve, and multiplexing for qPCR assays. SYBR Premix Ex Taq II (Tli RNaseH Plus) is designed for high speed, high sensitivity qPCR. Premix limits primer dimers, nonspecific amplification and inhibition from residual mRNA. Superior specificity and fidelity than.